Oligonucleotide Synthesis
Advance your research with our high-quality, custom-designed oligonucleotides
Oligonucleotide Synthesis
Advance your research with our high-quality, custom-designed oligonucleotides
Targeted, Potent, and Reliable RNAi Tools for Research and Therapeutic Development
We specialize in the design and synthesis of high-quality, custom oligonucleotides tailored to your research goals. Regardless of your model system or delivery vehicle, our synthesis services are built to deliver potency, specificity, and flexibility at every stage of development.
Precision-Engineered siRNAs and ASOs for Functional Studies and Therapeutics
Small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs) are powerful tools for gene silencing, each offering distinct mechanisms of action. siRNAs, typically 19–23 nucleotides long, mediate RNA interference (RNAi) by directing the cleavage of complementary mRNA. ASOs, on the other hand, are single-stranded oligonucleotides that bind target RNA to modulate splicing or trigger degradation via RNase H. With their high specificity and versatility, both siRNAs and ASOs are essential for probing gene function, validating drug targets, and advancing RNA-based therapeutics.
We synthesize siRNAs, ASOs, and other custom oligonucleotides with optimal design parameters to maximize silencing efficiency and minimize off-target effects, using our proprietary in-house algorithms.
siRNA and ASO Applications
- Gene function studies (in vitro/in vivo)
- High-throughput loss-of-function screens
- Disease modeling and drug screening
- Knockdown of "undruggable targets"
- Preclinical validation
siRNA and ASO Applications
- Gene function studies (in vitro/in vivo)
- High-throughput loss-of-function screens
- Disease modeling and drug screening
- Knockdown of "undruggable targets"
- Preclinical validation
Scalable Production for Any Stage of Research
We support projects at all scales—from pilot experiments to large-scale leads validation studies:
- Mini-scale (e.g., 1-10 nmol) — ideal for screening and validation
- Mid-scale (e.g., 10- 200 nmol) — optimal for extended in vitro testing
- Large-scale (e.g., 0.2–2umol) — for delivery studies, in vivo testing, or formulation trials
If you’re unsure what scale is most appropriate for your project, our scientific team is happy to consult with you directly. If we are unable to synthesize at the scale needed for your project, we can coordinate synthesis with our global partners.
See below for more information.
Full-Service Customization
We offer custom conjugation of synthesized siRNAs and antisense oligonucleotides (ASOs) to support a wide range of research and therapeutic applications. Whether you’re aiming for improved delivery, targeted cellular uptake, or optimal knockdown with minimal off-target effects, our team can design and incorporate conjugates tailored to your platform’s specific needs.
Common Conjugation Types
- Cholesterol – Enhances cellular uptake through passive membrane association
- DCA (docosanoic acid) – Facilitates lipophilic conjugation to support delivery via hydrophobic interactions
- GalNAc (N-acetylgalactosamine) – Enables hepatocyte-specific targeting via ASGPR, ideal for liver-directed therapies
- Biotin – Useful for affinity-based applications such as pull-downs
- Cy3 / Cy5 – Fluorescent dyes for visualization, localization, and semi-quantitative analyses
- Primary amino group – For custom chemical conjugations
Conjugates can be incorporated at either the 5′ or 3′ end of the oligonucleotide, depending on your preferred orientation and application needs.
Chemical Modifications & Customization
Incorporating chemical modifications enhances nuclease resistance, reduces immunogenicity, and optimizes silencing efficiency.
Available Backbone & Sugar Chemistries
- Phosphorothioate (PS) Linkages — Improves resistance to exonucleases and endonucleases
- 2'-O-Methyl (2'-OMe) — Reduces immunogenicity and increases stability
- 2'-Fluoro (2'-F) — Enhances target affinity and degradation resistance
- 2'-O-Methoxyethyl (MOE) — Extended in vivo half-life
- Locked Nucleic Acids (LNA) — Increases thermal stability and binding affinity
- 5' vP (5'-Vinylphosphonate) — Improves in vivo tissue accumulation and efficacy
While we primarily synthesize siRNAs and ASOs, we welcome custom requests for alternate oligo formats and chemistries. If you have a specialized application or novel construct in mind, our team is happy to collaborate and design a solution tailored to your project.
Quality You Can Trust
We guarantee purity, performance, and reproducibility with every batch. All synthesized oligos undergo stringent QC, including:
- Lyophilized shipment for long-term stability or pre-dissolved at a “ready to use” concentration
- RNase-free processing and duplex annealing
- HPLC and Mass Spec verification
Quality You Can Trust
We guarantee purity, performance, and reproducibility with every batch. All synthesized oligos undergo stringent QC, including:
- Lyophilized shipment for long-term stability or pre-dissolved at a “ready to use” concentration
- RNase-free processing and duplex annealing
- HPLC and Mass Spec verification
Customizable Delivery Formats
We can deliver our oligos in the following formats:
- As single strands (desalted or purified)
- As duplexed siRNA (quantified and annealed)
Ordering Information
We recommend providing the following when placing a request:
- Target gene and species
- Isoforms of interest (if applicable)
- Strand lengths
- Desired sequence(s) or target region
- Analytical methods
- Modifications or labels
- Quantity requirements
Not sure what you need? We offer complimentary design consultations for all new synthesis clients.
Extended Synthesis Capabilities & Global Sourcing
While we specialize in the in-house synthesis of siRNAs and ASOs with a defined set of modifications and conjugates, we understand that certain projects may require unique chemistries or production at scales beyond our current capabilities. We can source custom constructs that fall outside our direct manufacturing capabilities through established partnerships with a network of trusted global oligonucleotide suppliers.
If your project requires:
- Uncommon backbone or sugar modifications
- Specialized linkers or conjugation strategies
- Synthesis scales beyond our current capacity
Our team can coordinate the entire process—from sequence design and vendor communication to shipment and downstream screening at our lab. This ensures scientific continuity, reliable turnaround times, and data quality that meets our internal standards.
We remain your single point of contact, and we handle all technical review, QC confirmation, and formatting for follow-up evaluation in your chosen in vitro or in vivo system.
Precision Oligonucleotide Synthesis
ADViRNA delivers custom oligo synthesis powered by validated design algorithms, with fast turnaround and pricing that fits your budget.
Precision Oligonucleotide Synthesis
ADViRNA delivers custom oligo synthesis powered by validated design algorithms, with fast turnaround and pricing that fits your budget.